CENTRE FOR ENVIRONMENT, FISHERIES AND AQUACULTURE SCIENCE
CEFAS26-87 ITT for eDNA analysis of samples from Beyond the Reef survey
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https://atamis-9529.my.site.com/s/Welcome All bids to be submitted via the above eProcurement system.
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This sits in the upper-middle of the Engineering & Architecture band — a substantial contract for the sector. Based on 33,646 valued Engineering & Architecture tenders in our corpus.
The Blue Belt Programme is the UK Government’s flagship marine conservation programme, working with a number of the UK Overseas Territories to safeguard more than four million square kilometres of the World’s oceans.
Earlier this year, the Blue Belt Programme conducted its first offshore research expedition ‘Beyond the Reef’ to the Caribbean UK Overseas Territories of Anguilla, Cayman Islands and Turks and Caicos Islands.
As a component of the ‘Beyond the Reef’ survey, Cefas undertook water sampling, yielding 417 filters (348 samples, 69 field negatives).
Our overall aim is to identify the composition and distribution of teleost fishes and elasmobranchs around Anguilla, and the Turks and Caicos Islands in the Caribbean.
We require a Supplier to process these samples using a metabarcoding workflow and provide us with the raw sequence data.
As we are aiming to publish this research, we require a high level of transparency on, and input into, the laboratory procedures used.
Full specification details can be found in the Bidder Pack.
Services required include: - Extract DNA from the Sterivex (0.22 µm; PES) filters (348 total; triplicated per sampling station/depth; 4 L filtered total) and field negatives (69; one per station) provided by Cefas. - PCR amplify a ~170 bp fragment of the 12S rRNA region, with two primer sets: one targeting teleost fishes (MiFish-U) and another targeting elasmobranchs (MiFish-E). - Pooled PCR products will then be indexed. - Sequence the library(s) on a suitable High Throughput Sequencing Platform, e.g.
NextSeq 1000 P1 XLEAP-SBS, to an appropriate level of sequence depth. - Provide Cefas with demultiplexed paired-end read sequence data.
Contract is expected to start 1 September 2026 with delivery completed by 1 December 2026.
Some flexibility can be provided where essential, up to 1 March 2027.
What the supplier must deliver
We require a Supplier to process these
We require a Supplier to process these samples using a metabarcoding workflow and provide us with the raw sequence data.
Provide Cefas with demultiplexed paired-end read sequence
Provide Cefas with demultiplexed paired-end read sequence data.
Contract is expected to start 1 September
Contract is expected to start 1 September 2026 with delivery completed by 1 December 2026.
Derived from the notice text — always confirm against the original documents.
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- OCID
- ocds-h6vhtk-06b6b0
- Stage
- tender · Open
- Source
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- Buyer ref
- 056980-2026
Contains public sector information licensed under the Open Government Licence v3.0. Source data © Crown copyright.
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