Board of Trustees of the Royal Botanic Gardens Kew
RBGKEW1066 Long-Read Ash Genome Sequencing
This is a contract result notice, not an open opportunity. Details from the official award data.
This sits in the lower-middle of the Engineering & Architecture band — a mid-scale opportunity. Based on 33,646 valued Engineering & Architecture tenders in our corpus.
RBG Kew wishes to appoint a supplier to individually sequence the whole genomes of up to 50 European ash trees (Fraxinus excelsior) at a minimum of 15x whole genome coverage via a long-read technology.
Fraxinus excelsior has a 1C genome size of 880 Mbp.
RBG Kew intends to supply total genomic DNA for each tree with the following metrics: minimum of 20ug DNA; minimum concentration of 30ng/ul (as measured with a Quantus flurometer dsDNA assay); at least 80% of the sample in fragments with a minimum length of 40kb; minimum OD260/280 of 1.7; minimum OD260/230 of 1.7.
Based on a fixed budget of £57,000 GBP (excluding VAT), we wish to maximise: firstly, the number of trees sequenced at a minimum of 15x whole genome coverage (minimum of 13.2 gigabases of data per sample); secondly, in the event that all 50 trees can be sequenced to at least 15x (minimum of 13.2 gigabases of data per sample), the total amount of data in gigabases for each of the 50 trees.
You are hereby invited by the Board of Trustees of the Royal Botanic Gardens, Kew to submit a tender to meet the Customer's requirements in accordance with the specification and associated documents as contained within this tender pack.
Tender documents can be accessed via: https://defra.bravosolution.co.uk https://defra.bravosolution.co.uk/go/08518853018185EF98F9 itt_10303 project_36622 Title:- RBGKEW1066 Long-Read Ash Genome Sequencing 2022 Suppliers will be required to register with BRAVO, if not already registered.
All tender documents can only be accessed via BRAVO
What the supplier must deliver
RBG Kew intends to supply total genomic
RBG Kew intends to supply total genomic DNA for each tree with the following metrics: minimum of 20ug DNA; minimum concentration of 30ng/ul (as measured with a Quantus flurometer dsDNA assay); at least 80% of the sample in fragments with a minimum length of 40kb; minimum OD260/280 of 1.7; minimum OD260/230 of 1.7.
Suppliers will be required to register
Suppliers will be required to register with BRAVO, if not already registered.
Derived from the notice text — always confirm against the original documents.
Make the case to bid
Reveal who to approach at Board of Trustees of the Royal Botanic Gardens Kew, and generate a go-to-market strategy from their news, accounts and people.
Ask me about this tender, the buyer, who wins their work, or whether you should bid.
- OCID
- 6c949a0e-ddd3-4150-b43b-b7b3ff0e6e73
- Stage
- contract · Contract
- Source
- Contracts Finder
- Buyer ref
- RBGKEW1066 Long-Read Ash Genome Sequencing
Contains public sector information licensed under the Open Government Licence v3.0. Source data © Crown copyright.
Who wins this kind of work
The suppliers and buyers around this opportunity — drawn from official award data. Drag to orbit; click a node to explore.
Top suppliers & buyers in Engineering & Architecture
Board of Trustees of the Royal Botanic Gardens Kew’s tender network
Assembling the network…