Cryogenic Sample Preparation Equipment

Introduction to the procurement.

Mass spectrometry imaging (MSI) methods provide a powerful platform for high-throughput, high-resolution and high-sensitivity label-free 3D chemical analysis. However, there are critical limitations for the analysis of biological samples. The cryogenic sample preparative equipment, we are seeking to purchase, will expand our existing MSI infrastructure and enable us the development of measurement methodologies tailored specifically to biological samples (bacteria, bacterial biofilms, cells, tissues etc.) The equipment will significantly enhance our exiting capabilities, placing us in the position to pioneer the development of MSI metrology and methodologies specifically tailored to study biological samples such as bacteria, bacterial biofilms, cells and tissues. The system will enable imaging of cryogenically prepared samples with unprecedented detail and resolution by our unique instrumental capabilities, including the revolutionary 3D OrbiSIMS.

There is a gap in our infrastructure, which have to be filled in order to provide a comprehensive instrumental set-up, uniquely suited to study biological samples. This includes: Sample cryo-preparation system, including high pressure freezer, sample plunger and freeze substitution system; these capabilities will enable preparation and measurements of bacterial samples in frozen-hydrated state by high-vacuum of MS instruments. The immobilisation of water reduces ultrastructural reorganisation and the loss or translocation of exo-/endogenous molecules, which occurs with dehydration. Our revolutionary 3D OrbiSIMS instrument is already equipped with state-of-the-art cryo sample handling interface (Leica) allowing direct use of cryogenic samples and sample interchange between other cryo- instruments equipped with the Leica system. Compatible sample preparation capabilities will allow us to develop methodologies and to perform studies in a highly controlled and efficient manner, ensuring sample stability and minimal sample handling.